Oligonucleotide

Accelerating Oligonucleotides Discovery and Development Programs

With a commitment to innovation and excellence, Aragen offers a comprehensive suite of Oligonucleotides discovery and development services that encompass synthesis, purification, characterization, and more. We have a state-of-the-art laboratory for synthesis of simple to highly complex oligonucleotides for diverse applications viz. ASO, siRNA, miRNA, Aptamer, and other niche applications. A highly talented and experienced scientific team to handle modern automatic synthesis, purification, and characterization of modified oligonucleotides.

Service Offerings:

• DNA Oligonucleotides
• RNA Oligonucleotides
• Synthesis of customized building-blocks for oligonucleotide synthesis

Process flow for synthesis of oligonucleotides

Project evaluation

Comprehensive analysis of the project feasibility and goals by our experts. Estimation of raw material requirements, costs, and timelines

Purification

Reverse-phase HPLC purification, Anion exchange purification, Desalting

Delivery

Sharing of final analytical data Shipping of final product according to your request

Project request

Customized discovery and synthesis requests from researchers across the globe

Synthesis

Synthesis scale: 1 μmol – 100 μmol, Yield scale: 1 mg -100 mg (deliverable quantities), oligonucleotide lengths: 5 – 10 mer

Quality assurance

RP-HPLC purity check (UV), MS confirmation, Quantification based on A260 (UV-mg)

Our portfolio

• Nucleotides:
  • Standard: DNA, RNA, 2’OMe-RNA, 2’F-RNA, 2’MOE-RNA, LNA
  • Custom synthesized sugar and base modified nucleotides
  • Linkers: PO/PS/P-Me/P-Ms chemistry
• Scale:
  • Dynamic synthesis scale flexibility: 1-100 umol
  • Yield scale: 1-100 mg
• Applications:
  • Bulk-scale primers and probes
  • Small oligonucleotides (5-15 nt)
  • Antisense oligonucleotide (16-22 nt)
  • siRNAs (20-35 nt)
  • Aptamers (35-75 nt)
  • Long oligonucleotides for RNA editing and other applications
• Modification:
  • 3’/5’/int
• Functional units:
  • Fluorophores, quenchers, cholesterol, biotin,
  • Digoxigenin, tris-galnac, phosphate,
  • 5’-vinyl-phosphate, lipids spacers and others
• Attachment units:
  • Amine/thiol/alkyne/azide
• Solution phase conjugation:
  • Acid-amine coupling
  • Thiol-maleimide coupling
  • Azide-alkyne coupling
• Cross department services
  • In-house synthetic expertise to synthesize modified bases, linkers, and sugar motifs
  • Peptide-oligonucleotide conjugation
  • Protein/antibody-oligonucleotide conjugation
  • Oligonucleotide bioassays: tox studies, efficacy studies

Oligonucleotide Infrastructure

Our in-house synthetic expertise utilizes fully automated state-of-the-art equipment that ensures everything runs like clockwork.

Synthesis

• H-16 Oligonucleotide Synthesizer by K&A with digital SCADA

Purification 

• AKTA Pure 150M3 for ion exchange purification
• AktaFlux-S semi-automated TFF system
• Shimadzu Nexera Preparative HPLC 

Analysis:

• NanoDrop One microvolume UV-Vis spectrophotometer
• Thermo Orbitrap Exploris 240 Mass Spectrometer coupled with Vanquish UPLC
• Waters Acquity H-class Bio UPLC system with HDMS for robust separations in high ionic buffers

Sample processing

• Centrifugal evaporator with temperature range of room temperature to 80°C
• Refrigerated Centrifuge with cooling temperature range of 4 °C to 40 °C
• Lyophilizer