Bacterial Expression System
- The E. coli expression system is one of the most widely used platforms for producing recombinant proteins because of ease of use, fast growth, cost – effectiveness, robust yields, and well – characterized genetics.
- Exhaustive expression screen strategies for enhanced soluble expression, improved disulfide bond formation, proper protein folding, etc.
- Diverse expression strains – BL21(DE3), BL21(DE3)pLysS, BL21-AI, SHuffle, Rosetta, ArcticExpress (D3), etc.
- In vitro protein refolding from inclusion bodies.
Capacity: Up to 100 L using shake flasks.
Timeline: Expression and Purification: 1 – 2 weeks*.
Yeast Expression System
- The yeast expression system has been used for producing biologics such as insulin, hepatitis vaccines, and therapeutic enzymes.
- Pichia pastoris expression system offers scalable, sustainable, cost-effective solutions for producing high-quality recombinant proteins with complex PTMs.
- Expression screen strategies towards high – yield secretory expression.
- Choice of expression strains (X-33, KM71H, GS115, PichiaPink)
- Stringent selection of expression colonies.
Capacity: Up to 10 L using shake flasks; Upto 5 L using BioFlo 120.
Timeline: Strain generation, expression and purification: 3 – 4 weeks*.
Insect Cell / Baculovirus Expression System
- Insect cells offer an efficient and versatile platform for recombinant protein production, especially recombinant protein complexes with advantages like robust post- translational modifications (PTMs), high yield and scalability.
- Robust expression screen strategies including
- Choice of expression strains (Sf9, Sf21, Hi-Five)
- Secretory and intracellular expression.
- Diverse baculovirus generation methodologies.
- Modulating virus infection and expression conditions.
Capacity: Shake flasks up to 65 L; Wave bioreactor- 2 x 25 L.
Timeline: Bacmid & virus generation, Expression and Purification: 5 – 6 weeks*.
Mammalian Expression Systems
- The mammalian expression system is the undisputed leader in recombinant protein production when precision, functionality, and therapeutic efficacy matter most.
- This system delivers the highest-quality proteins, ensuring success in the most demanding applications with human-like post-translational modifications, superior protein functionality and stability, proven success in biologics, scalability for industrial production, and critical for antibody and vaccine development.
- Robust expression screen strategies including
- Choice of expression cell lines (HEK293F, Expi293, ExpiCHO and CHO-S).
- Secretory and intracellular expression.
- Additional optimization parameters: media, feed, temperature, time of expression.
Capacity: Shake flasks up to 80 L; Wave bioreactor- 2 x 25 L.
Timeline: Expression and Purification: 2 weeks* (for HEK293); 2 – 3 weeks* (for CHO).
*Excluding gene synthesis
Recombinant Protein Purification Services
For biotech and pharmaceutical companies seeking the best in recombinant protein purification, Cell and Protein Sciences at Aragen provides top-tier quality, and scalability. With years of expertise, customizable services, and commitment to excellence, Aragen is your trusted partner for unlocking the full potential of your recombinant proteins.
Why Choose Aragen for Your Recombinant Protein Purification Needs?
- Industry-Leading Expertise: Cell and Protein Sciences has a proven track record of delivering 1000+ purified recombinant proteins for leading biotech, Pharmaceutical, Agrosciences, and Animal health companies worldwide – meeting the highest standards for research and development requirements.
We have extensive experience in producing a diverse array of proteins such as cytokines, enzymes, kinases, proteases, animal vaccines, extra-cellular domains (ECDs), E3 Ligases, antibodies- monoclonal antibodies (mAbs), bi-specific (BsAbs), tri-specifics, scFvs, Fabs, nanobodies, etc.
- Diverse Tag-Based Purification Solutions: We provide various choices of affinity tags such as His, Strep, GST, MBP, FLAG, Fc with an option of tag removal by proteolytic digestion such as TEV, SUMO, PreScission Protease, Thrombin, Factor Xa, etc.
- Custom-Tailored Purification Solutions: We understand that each project is unique. That’s why we offer fully customizable recombinant protein purification services, including affinity (Ni-NTA, Strep, GST, amylose, FLAG, Protein A/G/L), ion-exchange, hydrophobic interaction, size-exclusion, and mixed-mode chromatography techniques.
- High Yield, High Purity, Guaranteed: Our comprehensive purification strategies deliver recombinant proteins with high yield and exceptional purity (> 95%). We ensure that your proteins are biologically active, stable, and ready for downstream applications.
- Low Endotoxin proteins: We can deliver antibodies and reagent proteins with low endotoxin levels (< 1 EU/mg) to meet your specific downstream requirements.
Quality Control: Our rigorous quality control includes in-depth characterization of purified recombinant proteins through SDS-PAGE, Western blot, mass spectrometry, and analytical HPLC/UPLC, ensuring that your protein meets all necessary specifications. For more details see our Protein Characterization services.
