Recombinant Protein Production

Bacterial Expression System

• The E. coli expression system is one of the most widely used platforms for producing recombinant proteins because of ease of use, fast growth, cost – effectiveness, robust yields, and well – characterized genetics.
• Exhaustive expression screen strategies for enhanced soluble expression, improved disulfide bond formation, proper protein folding, etc.
• Diverse expression strains – BL21(DE3), BL21(DE3)pLysS, BL21-AI, SHuffle, Rosetta, ArcticExpress (D3), etc. 
• In vitro protein refolding from inclusion bodies.

Capacity: Up to 100 L using shake flasks. 

Timeline: Expression and Purification: 1 – 2 weeks*.

Insect Cell / Baculovirus Expression System

• Insect cells offer an efficient and versatile platform for recombinant protein production, especially recombinant protein complexes with advantages like robust post- translational modifications (PTMs), high yield and scalability.
• Robust expression screen strategies including 
  • Choice of expression strains (Sf9, Sf21, Hi-Five) 
  • Secretory and intracellular expression.
  • Diverse baculovirus generation methodologies.
  • Modulating virus infection and expression conditions.

Capacity: Shake flasks up to 65 L; Wave bioreactor- 2 x 25 L. 

Timeline: Bacmid & virus generation, Expression and Purification:  5 – 6 weeks*.

Recombinant Protein Purification Services

For biotech and pharmaceutical companies seeking the best in recombinant protein purification, Cell and Protein Sciences at Aragen provides top-tier quality, and scalability. With years of expertise, customizable services, and commitment to excellence, Aragen is your trusted partner for unlocking the full potential of your recombinant proteins.

Why Choose Aragen for Your Recombinant Protein Purification Needs?

• Industry-Leading Expertise: Cell and Protein Sciences has a proven track record of delivering 1000+ purified recombinant proteins for leading biotech, Pharmaceutical, Agrosciences, and Animal health companies worldwide – meeting the highest standards for research and development requirements.
  We have extensive experience in producing a diverse array of proteins such as cytokines, enzymes, kinases, proteases, animal vaccines, extra-cellular domains (ECDs), E3 Ligases, antibodies- monoclonal antibodies (mAbs), bi-specific (BsAbs), tri-specifics, scFvs, Fabs, nanobodies, etc.

• Diverse Tag-Based Purification Solutions: We provide various choices of affinity tags such as His, Strep, GST, MBP, FLAG, Fc with an option of tag removal by proteolytic digestion such as TEV, SUMO, PreScission Protease, Thrombin, Factor Xa, etc.
• Custom-Tailored Purification Solutions: We understand that each project is unique. That’s why we offer fully customizable recombinant protein purification services, including affinity (Ni-NTA, Strep, GST, amylose, FLAG, Protein A/G/L), ion-exchange, hydrophobic interaction, size-exclusion, and mixed-mode chromatography techniques. 
• High Yield, High Purity, Guaranteed: Our comprehensive purification strategies deliver recombinant proteins with high yield and exceptional purity (> 95%). We ensure that your proteins are biologically active, stable, and ready for downstream applications.
• Low Endotoxin proteins: We can deliver antibodies and reagent proteins with low endotoxin levels (< 1 EU/mg) to meet your specific downstream requirements.
• Quality Control: Our rigorous quality control includes in-depth characterization of purified recombinant proteins through SDS-PAGE, Western blot, mass spectrometry, and analytical HPLC/UPLC, ensuring that your protein meets all necessary specifications. For more details see our Protein Characterization services.