Antibody–Drug Conjugate (ADC) Discovery & Bioconjugation Solutions

Antibody Discovery and Engineering

• Hybridoma-based antibody discovery
• Mammalian expression of antibody formats
• Antibody engineering 
• Antibody production and purification for conjugation studies

Bioconjugation Technologies

• Chemical Conjugation: Cysteine conjugation, Lysine conjugation, ThioMab conjugation, Click chemistries.
• Enzymatic Conjugation: Microbial transglutaminase (MTGase),  Sortase-mediated conjugation, SpyLigase systems

High-Potency Payload and Linker Expertise

Our platform supports synthesis and handling of ADC payloads and linkers, including:

• Auristatins
• Maytansinoids
• Pyrrolobenzodiazepines (PBDs)
• Topoisomerase inhibitors
• DNA/RNA-targeting payloads

Payload handling is performed in high-containment environments designed for potent compounds.

ADC Characterization and Analytical Services

• Drug–Antibody Ratio (DAR) determination
• LC-HRMS analysis for ADC composition
• aSE-HPLC (Purity and aggregate analysis)
• SDS-PAGE
• Free payload quantification
• Thermal stability assessment
• Antibody-Antigen binding characterization using ELISA, SPR, and FACS

High-Containment Infrastructure

ADC discovery is performed in OEB-5 laboratories designed for safe handling of highly potent compounds:

• Isolator-based suites with OELs < 1 µg/m³ and contained reactor systems.
• Dedicated HVAC & air-handling systems with HEPA filters, wet scrubbers, and detox protocols to prevent cross-contamination.
• Specialized handling areas: RALFs and Class-II BSCs for handling of  final ADCs.
• Segregated waste management and continuous environmental monitoring.

These systems ensure safe, controlled, and contamination-free ADC discovery workflows.

Why Partner with Us for ADC Discovery?

ADC discovery demands specialized expertise, speed, and rigorous safety to handle highly potent compounds. Aragen delivers integrated capabilities to accelerate discovery programs.

• Rapid & Flexible Synthesis: Fast turnaround for linker–payload synthesis and SAR campaigns with seamless scale-up from mg to g and custom chemotype synthesis.
• Integrated Project Execution: Dedicated cross-functional teams ensuring efficient coordination from route scouting to manufacturing.
• Safety-First Culture: Continuous training and robust containment practices ensuring the highest standards in process safety and occupational health.