The nature of the molecule, the biology being investigated, and the overall requirements for throughput and yield, determine the in vitro testing decision. Aragen skilled scientists work with customers to develop and implement assays best suited for their specific research requirements. We offer a diverse portfolio of cell-based and biophysical assays that enable the development and characterization of new therapeutics, based on the physical characterization of the protein (expression, size, weight, binding), as well as biological activity in mammalian cells, and ultimately, in animals.
Our cell biology team has extensive experience in developing cell-based assays for determining mechanisms of action, evaluating potential therapeutics, and screening small libraries of compounds. The protein sciences team provides a range of analytical methods using multiple assay platforms to characterize therapeutic, diagnostic or reagent-grade proteins.
Areas of Expertise
Assay Type
Immunology
Immunophenotyping
Lymphocyte subset analysis
Toxicity assays – ADCC, ADCP, CDC
Cell activation
Fc receptor binding
Mixed lymphocyte reactions
Cytotoxic T lymphocyte (CTL) and natural killer activity assays
Treg, NK cell, macrophage assays
Multiplex cytokine profiling
ELISpot
Immune function assays
ELISA
Live-cell imaging using the IncuCyte S3
Oncology
Apoptosis
Cell proliferation (BrdU, MTT)
Antibody-target affinity
In vitro angiogenesis
Signaling
Phospho-flow
Reporter gene assays
Migration assays
Custom assays
Primary Cell
Immunology assays
HUVEC, HMVEC, VSMC, RPTEC
Other (a variety of primary immune cells isolated from whole blood or buffy coats)
Fibrosis assays
Epithelial-to-mesenchymal transition (EMT)
Fibroblast-myofibroblast transformation (FMT)
Co-culture assay
Precision-cut tissue slice assay
SARS-CoV-2 screening assay
Pseudovirus system
Other
Genotyping
siRNA testing
Chemotaxis assays: employ a Transwell containing a membrane with a defined pore size; run in 96-well format
Cell migration and cell invasion assays: employ ECM degradation and proteolysis
