Aragen provides customized gene synthesis services. We synthesize the DNA sequences chemically according to the customer request that includes the DNA sequences that is difficult to clone, including repeated sequence, GC rich sequences, hairpin structure, same nucleotide repeated sequence etc. We guarantee 100% sequence accuracy and free codon optimization service to save project time
Aragen understands the unique requirement of each client. Our molecular biology experts help the client to construct a completely customized vector. Using our de novo DNA synthesis capability, we will make any fragments that are required for the vector. Our know-how and experience help us provide premium quality molecular biology and protein expression services to clients from biotech and pharmaceutical companies.
Identifying a strong host cell line platform is a critical factor to produce an efficient biologic. Several factors are to be considered before selecting the host cell line, such as particular project requirements and the biologic being anticipated. Aragen will assist its customers with constant interactions during the early stages by selecting the host cell line after considering all the physical and chemical properties of the protein. Some of our host cell line platforms are CHO-DG44, CHO-GS, HEK293, SP2/0, and NS0. These platforms are very popular among our clients for expressing a broad range of molecules, including antibodies, bi-specifics, and fusion proteins. Aragen’s Cell Line Development (CLD) expertise using a royalty-free and regulatory-friendly platform, makes Aragen your partner of choice
Aragen has full capabilities in transient and stable expression for both small and large scales. Our expression systems and scales are described in tables below.
| Protein Production: Transient Transfection | |
|---|---|
| Transfection system | Lipid Base |
| Cell Type | HEK293, CHO-S, Expi-CHO, Expi-HEK293 |
| Scale | 30mL to 10L in Shaker Flask |
| 5L to 20L in Wave | |
| Capacity | 20 Construct at a time |
| Dedicated E. Coli Lab. | Capacity up to 10L production with purification |
| Protein Production: Stable Cell Line | |
|---|---|
| Production run size from small scale ~2L to max 240L in Wave. | |
| Expertise in CHO, CHO-GS, CHO-S, Hybridoma. | |
| Production using Aragen’s STD process as well as customized Client’s process. | |
| Expertise in controlled Stirred Tank Bioreactor- 12X1L, 3X5L and 1X10L Bioreactor. | |
Aragen has established downstream platforms to help our customers to move quickly into products manufacturing phase. Our decade of experience had enabled us to offer customized and innovative solutions to meet the customers’ growing needs. We have optimized our downstream processes to remove impurities and increase yields with minimal steps and optimal recovery, resulting in more cost-efficient manufacturing process. Our analytical teams are fully integrated to ensure the product quality attributes. Our downstream development capabilities and Analytical capabilities are listed below.
| Protein Analytics | Quality Attribute | Platform |
|---|---|---|
| Protein concentration by A280 | Quantity | UV-Vis |
| A280, A320, Appearance | General appearance- Turbidity | |
| Free Thiol by Ellman’s Assay | Primary Structure- Free Thiol | |
| Enzyme Kinetics (Kcat, Kcat/Km) | Potency | |
| N-Glycan profiling by CE | Primary Structure – PTMs | SCIEX PA 800Plus |
| Charge variants by cIEF | Purity-charge variants | |
| Charge variants by CZE | Purity-charge variants | |
| Size variants by (Fragments) by CE-SDS | Purity Size varients | |
| LC-MS intact Mass (non-reduced, reduced,+/-PNGaseF) | Primary structure-MW | LC-MS/MS Agilent QTOF attached to nanoflow Chip-Cube or microflow UPLC (ESI) |
| LC-MS/MS PepMap | Primary structure-PTMs, Modifications | |
| PepMap UV | Identity | |
| N-Glycan profiling by RP-MS or HILIC-MS | Primary structure-PTMs | |
| Glycation by LC-MS/MS | Primary structure- Modifications | |
| Absolute mass of monomer and aggregates | MW-Size variants | SEC-MALS (Wyatt 18-angle MALS) |
| Tm (Fluorescence), Tagg (SLS), Size and PDI(DLS) | Thermal stability, Higher order structure (HOS) | Unchained Labs UNcleTM |
| Slab gel IE | Purity – Charge variants | Gel Electrophoresis |
| SDS Page (non-reduced and reduced) | Purity – Size variants | |
| Western blot | Identity | |
| Binding Kinetics (Kon, Koff, KD) | Potency, Identity | BioLayer Interferometry (BLI) ForteBio Octet RED Or Surface Plasmon Resonance (SPR) Biacore |
| FcR and C1Q binding | Half-life , Safet | |
| Protein concentration, antigen binding titer | Quantity | |
| Epitope Binning | Epitope Characterization | |
| Functional blocking | Potency, Identity | |
| Yes/No binding | Identity | |
| Binding ELISA | Potency | Molecular Devices SpectraMax |
| HCP ELISA (Cygnus | Safety | |
| Residual Protein A/G/L by ELISA | Safety | |
| Endotoxin by Limulus Amebocyte Lysate (LAL) | Safety | Charles River Endosafe |
| Bioburden, Sterility Test | Safety | Culturing |
